New mechanisms of nucleotide excision DNA repair coupled with transcription activation in human cells
A 30-month research engineer position funded by the Fondation ARC is open from November 2017 to work in the group “Genome Transcriptional Regulation” headed by Dr. Julie Soutourina at the Institute for Integrative Biology of the Cell (I2BC), Integrative Biology and Molecular Genetics Unit (SBIGeM) on the Saclay campus of the CEA (Paris region, France).
The research engineer/post-doctoral fellow will be in charge of the part of the on-going project on the new mechanisms of nucleotide excision DNA repair coupled with transcription activation using human cells as a model system. The project is based on our recent discovery of a novel Mediator role in transcription-coupled DNA repair. We aim to develop new concepts of the functional interplay between transcription and DNA repair, two fundamental processes, dysfunctions of which lead to serious human diseases including cancer.
The successful candidate will have a PhD in molecular biology with solid background knowledge in genetics and functional genomics. A prior experience in human cell biology is strongly required. We are seeking for highly motivated candidates interested in the deciphering how transcription and DNA repair are coordinated in human cells on the genomic scale. A previous experience in ChIP-sequencing approaches will be appreciated.
Interested candidates should contact Dr. Julie Soutourina at firstname.lastname@example.org and send a Curriculum Vitae, including past research experience and publication records, as well as a letter detailing their motivation and interest in our work. Applicants should also provide the names and contact details for two or three references and recommendation letters.
Selected references of the group:
Eychenne T., Novikova E., Barrault M.-B., Alibert O., Boschiero C., Peixeiro N., Cornu D., Redeker V., Kuras L., Nicolas P., Werner M., & Soutourina J. (2016) Functional interplay between Mediator and TFIIB in preinitiation complex assembly in relation to promoter architecture. Genes & Dev. 30, 2119-2132.
Eyboulet F., Cibot C., Eychenne T., Neil H., Alibert O., Werner M. & Soutourina J. (2013) Mediator links transcription and DNA repair by facilitating Rad2/XPG recruitment. Genes & Dev. 27, 2549-2562.
Carrière et al. (2012) Genomic binding of Pol III transcription machinery and relationship with TFIIS transcription factor distribution in mouse embryonic stem cells. Nucleic Acids Res. 40, 270-283.
Soutourina, J. et al. (2011) Direct RNA polymérase II – Mediator interaction required for transcription in vivo. Science 331, 1451-1454.
French Alternative Energies and Atomic Energy Commission (CEA)
Saclay Center ½ 91191 Gif-sur-Yvette Cedex
T. +33 (0)1 69 08 54 13 ½ F. +33 (0)1 69 08 47 12
Fundamental Research Division
Institute of Life Sciences Frédéric Joliot
Integrative biology and molecular genetics unit
Institute for Integrative Biology of the Cell
Genome Biology Department